what is a Strain fermentation equipment？

what is a Strain fermentation equipment？

Strain fermentation equipment (strain fermentation tank/bioreactor)Core application: In a sterile and enclosed environment, liquid bacterial expansion of microorganisms (such as Bacillus subtilis, lactic acid bacteria, edible fungi, organic fertilizer functional bacteria, etc.) is carried out, with precise temperature control pH、 Dissolve oxygen to obtain highly active bacterial liquid in a short period of time, commonly used in the production of microbial fertilizers, edible fungi, and microbial preparations.

Mainstream classification of strain fermentation equipment

1. Liquid strain fermentation tank (most commonly used, organic fertilizer/edible mushroom strain expansion)
Mechanical stirring fermentation tank: mainstream in the market. Tank body made of 304/316L stainless steel, equipped with jacket for cold and hot temperature control, stirring blade, sterile ventilation system, pH/dissolved oxygen probe, and PLC self-control; Can be sterilized by steam in place (actual sterilization). Seed tank (small volume primary expansion), main fermentation tank (large-scale cultivation), three-stage fermentation process: shaking bottle → seed tank → production tank.
Self suction fermentation tank: No need for air compressor, negative pressure suction of air by the stirring impeller simplifies the air system and is suitable for small and medium-sized aerobic bacteria.
Air lift fermentation tank: No mechanical stirring, relying on airflow to lift circulation, low energy consumption, not easy to pollute, suitable for shear sensitive bacterial strains.
Specifications: Laboratory 5L/10L; Pilot scale production of 50-500L; industrial production of 1 ton, 5 tons, 10 tons, and 30 tons.
2. Solid state bacterial fermentation equipment
Solid state fermentation tank/drum solid fermentation tank: low moisture solid substrate fermentation, producing solid microbial agents.
Organic fertilizer tank fermentation turning machine: Pay attention to differentiation – the turning machine is a raw material composting equipment, not a strain expansion tank. It adds expanded strains to animal manure for composting.
Box type closed fermentation tank: aerobic solid fermentation, temperature controlled aeration, used for the production of solid microbial agents.

Standard composition of strain fermentation equipment

1. Tank body: stainless steel, jacket (heating/cooling water circulation temperature control)
2. Mixing system: motor+mixing blade, breaking bubbles and increasing dissolved oxygen
3. Ventilation system: air compressor → refrigerated dryer → multi-stage air filter, providing sterile air (essential for aerobic bacteria)
4. Online sensors: temperature pH、 Dissolved oxygen DO, foam probe
5. Control system: PLC touch screen, automatic feeding, pH adjustment, defoaming, and curve recording
6. Auxiliary: steam sterilization pipeline, sight glass, sampling port, inlet and outlet, defoaming device, tail gas filter

Working principle of strain fermentation equipment

Add culture medium into the tank and sterilize with steam at 121 ℃; After cooling, sterile inoculation of bacterial strains; The control system maintains the optimal temperature and pH of the bacterial strain; continuously introduces sterile air and stirring to allow the bacterial strain to fully contact nutrients and oxygen, and rapidly proliferate; After fermentation, high concentration bacterial liquid (liquid bacterial species) is obtained by placing it in the tank.
Aerobic bacterial strains (Bacillus, actinomycetes, edible fungi): strong aeration and stirring;
Anaerobic bacteria (lactic acid bacteria): Non ventilated, enclosed anaerobic environment.

Application scenarios of strain fermentation equipment

1. Organic fertilizer industry: liquid propagation of functional bacteria such as Bacillus subtilis and Bacillus subtilis, producing liquid microbial agents and solid microbial fertilizers;
2. Edible mushrooms: liquid strains of shiitake mushrooms, shiitake mushrooms, and morel mushrooms;
3. Biological agents, aquatic EM bacteria, and feed probiotics;
4. Pharmaceuticals, enzyme preparations, yeast fermentation.

Key points for selecting bacterial fermentation equipment (reference for organic fertilizer bacterial production line)

1. Material: Production of bacterial fertilizer prioritizes 304 stainless steel; Choose 316L for high-end formulations;
2. Sterilization method: In place sterilization (actual sterilization) is more worry free and reduces bacterial contamination;
3. Supporting facilities: air compressor, air filtration system, steam boiler/electric heating, chiller;
4. Process: Small batch menu cans; Scaling up requires secondary/tertiary expansion of seed tanks and main fermentation tanks;
5. Control requirements: Simple production with optional simple electronic control; We recommend PLC online dissolved oxygen and pH automatic control for stable mass production.

List of supporting equipment for strain fermentation equipment

Air compressor, air storage tank, cold dryer, precision air filter, steam generator, water chiller, pipeline valves, sampling system, foam collection, bacterial liquid storage tank.